Ultrasound-Based Three-Dimensional Microangiography for Repeated Noninvasive Imaging of Neovascularization

Neovascularization plays an essential part in the progression of several diseases as well recovery processes such angiogenesis-guided neurogenesis after a spinal cord injury. To visualize and quantify neovascularization experimental setup, it is desirable to apply noninvasive, repeatable, harmless technique. Here, we newly developed ultrasound-based technique construct angiographies regenerating regeneration competent animal model, Mexican axolotl. We measured vessel volume fraction prior induction contusion transection injury repeated this measurement directly at 3, 6, 12, 63 days Although neither types resulted statistically significant differences relative sham-operated animals, there was increase over time all groups. Additionally, final point (63 injury) quantified with micro-CT imaging vascular perfusion contrast agent, confirming no difference between types. Ex vivo by significantly different from same point. This likely result incomplete agent before imaging, which supported subsequent histological evaluation. In summary, results suggest that angiographic procedure, demonstrate here, applicable noninvasive fashion longitudinal experiments circumventing limitations agent-dependent techniques.